唾液酸酶联合检测在阴道炎中的临床应用及意义 唾液酸苷酶阳性
[摘要] 目的 探讨唾液酸酶联合检测在阴道炎中的应用价值和意义。 方法 回顾性分析我院2010年12月～2011年5月收治的538例阴道炎患者的临床资料，所有患者均进行阴道分泌物白带三项检测[过氧化氢（H2O2）浓度、唾液酸酶、白细胞酯酶]，快速诊断阴道炎，并与传统的Amsel法检出结果比较。 结果 以Amsel法为标准，H2O2联合唾液酸酶检测的符合率、敏感性、特异性分别96.1%、83.2%、98.9%，唾液酸酶单项检测的符合率、敏感性、特异性分别为94.4%、88.4%、95.7%， H2O2联合唾液酸酶检测对阴道炎的诊断优于唾液酸酶单独检测，差异有统计学意义（P 4.6检出有较好的一致性，白细胞酯酶阳性检出与清洁度 > Ⅲ度检出有较好的一致性。 结论 唾液酸酶联合检测对阴道炎的诊断有较高的临床价值，为提高诊断率，在临床检验时可结合阴道分泌物的清洁度、滴虫、霉菌检查作为常规检查项目。
[关键词] 唾液酸酶；联合检测；阴道炎；临床应用
[中图分类号] R711.31 [文献标识码] B [文章编号] 1673-7210（2012）07（b）-0073-02
The clinical application and significance of sialidase joint detection in vaginitis
MA Xinru
Maternal and Child Care Service Centre of Qingyuan County, Hebei Province, Qingyuan 071100, China
[Abstract] Objective To investigate the clinical application value and significance of sialidase joint detection in vaginitis. Methods Clinical data of 538 cases of patients with vaginitis in our hospital form December 2010 to May 2011 were analyzed retrospectively. All patients underwent vaginal secretions leucorrhea three items detection (concentration of hydrogen peroxide, sialidase, leukocyte esterase) and were rapid diagnosis of vaginitis, which were compared with results of the conventional Amsel method. Results Taken Amsel method as the standard, the coincidence rate, sensitivity, specificity of H2O2 combined with sialidase detection were 96.1%, 83.2%, 98.9%; the coincidence rate, sensitivity, specificity of sialidase single detection were 94.4%, 88.4%, 95.7% respectively; H2O2 combined with sialidase detection in the diagnosis of vaginitis was better than that of sialidase single detection (P 4.6 detection. Leukocyte esterase positive detection had better consistency with cleanliness > Ⅲ degree detection. Conclusion Sialidase joint detection has high clinical value in diagnosis of vaginitis. In clinical inspection it can be combined with vaginal cleanliness, trichomonas, mold examination as routine examination items, in order to improve the diagnosis rate.
[Key words] Sialidase; Joint detection; Vaginitis; Clinical application
阴道炎是妇科门诊常见病，是阴道黏膜及黏膜下结缔组织的炎症，主要分为细菌性阴道炎（BV）、念珠菌性阴道炎、滴虫性阴道炎等[1]。唾液酸酶是BV致病菌所产生的特异性酶，最近的研究证实，阴道分泌物中唾液酸酶活性与BV之间存在一定的量的关系，其活性与早产、胎膜早破、低体重儿等一系列不良妊娠后果有直接关系[2]。正常妇女的阴道分泌物中含很少量的唾液酸酶或不含唾液酸酶，BV妇女阴道分泌物中的唾液酸酶活性明显高于正常妇女。因此，通过检测阴道分泌物中唾液酸酶活性可以诊断患者是否患有BV。本研究通过回顾性分析我院2010年12月～2011年5月收治的538例阴道炎患者的临床资料，探讨唾液酸酶联合检测在阴道炎中的应用价值和意义。