鸟苷酸环化酶受体的配体 环氧化酶-1、环氧化酶-2、Fas配体在门脉高压大鼠胃组织中的表达及意义

　　[摘要] 目的 利用门脉高压大鼠模型观察环氧化酶-1（cyclooxygenase-1，COX-1）、环氧化酶-2（cyclooxygenase-2，COX-2）、Fas配体（Fas ligand，Fas-L）在其胃组织的表达情况并探讨其在门脉高压性胃病中的意义。 方法 雄性SD大鼠20只随机分为实验组和对照组，实验组采用门静脉半结扎法制作门静脉高压大鼠模型并于模型制作成功两周后测量门静脉压力；对照组为假手术组，单纯游离门静脉后缝合切口并于两周后测门静脉压力；测门静脉压后分别取实验组与对照组大鼠胃组织，运用免疫组织化学方法观察COX-1、COX-2、Fas-L的表达情况。 结果 实验组大鼠门静脉压力明显大于对照组大鼠门静脉压力（P ＜ 0.05）；实验组大鼠胃组织中COX-1表达明显低于对照组（P ＜ 0.05）；COX-2的表达二者没有明显差别；Fas-L的表达明显增高（P ＜ 0.05）。 结论 COX-1一定程度上参与了门脉高压性胃疾病的形成，而COX-2可能并没有参与这一过程的实现。
　　[关键词] 环氧化酶-1；环氧化酶-2；Fas配体；门脉高压性胃病
　　[中图分类号] R573 [文献标识码] A [文章编号] 1673-7210（2012）07（c）-0016-03
　　Expression and significant of cyclooxygenase-1, cyclooxygenase-2, Fas ligand in the gastric tissue of portal hypertensive rats
　　GUO Zhikun GUO Jiansheng MIAO Jinyang
　　Department of General Surgery, the First Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030001, China
　　[Abstract] Objective To observe the expression of cyclooxgenase-1(COX-1), cyclooxgenase-2(COX-2) and Fas ligand (Fas-L) in the gastric tissue of portal hypertensive rats and investigate the significance in portal hypertensive gastropathy on the model of portal hypertensive rats. Methods Male SD rats were randomly divided into a experimental group and a contral group. The SD rats in experimental group were performed by partial portal vein ligation plus the ligation of the left adrenal vein and the portal vein pressure was measured in two weeks. The contral group was sham-operated, the same operation was performed with the exception that no ligature was placed after isolating the portal vein. The portal vein pressure was measured in two weeks. The expression of COX-1, COX-2 and Fas-L in the gastric tissue were observed after the portal vein measured by immunohistochemistry. Results The portal vein pressure of rats in the experimental group were significantly higher than the rats in the control group (P ＜ 0.05). The expression of COX-1 in the gatric tissue of portal hypertensive rats in experimental group was down regulated (P ＜ 0.05), however the expression of COX-2 in the gastric tissue these two groups were not significantly different between the experimental group, and the control group and the expression of Fas-L was upregulated in the experimental group (P ＜ 0.05). Conclusion The data indicate that the COX-1 certain extent in the portal hypertensive gastric disease formation, however COX-2 is probably not involved in this process.
　　[Key words] Cyclooxgenase-1; Cyclooxgenase-2; Fas ligand; Portal hypertensive gastropathy